rabbit anti sars cov2 n protein antibody  (Sino Biological)

Journal: PeerJ

Article Title: A novel one-step quick assay for detection of SARS-COV2 antibodies across mammalian species

doi: 10.7717/peerj.11381

Figure Lengend Snippet: (A) A strategy of direct visualization of antigen-antibody reaction via aggregation by protein A/G/L. Antigen is labeled with GFP. Protein A/G/L cross-links Ig Fc and/or light chain of all antibodies (IgG, IgM, IgA, IgE, and IgD) that bind antigen-GFP to form high fluorescence aggregates. Aggregating fluorescent antigen-antibody complexes simultaneously depletes background fluorescence to achieve a high sensitivity. (B) One-Step assay. Rabbit polyclonal antibodies against SARS-CoV2 N nucleocapsid proteins were diluted in human serum. Different amount of the rabbit antibodies (100 ng (C), 10 ng (D), 1 ng (E)) was incubated with the scN-GFP fusion proteins for 5 min to examine the detection limit of the one-step test. Blackwhite images show antigen-antibody aggregates, and green fluorescence of the aggregates is shown side by side. Bar: 100 um. Non-specific background of the antigen-antibody aggregates was examined after 5 day incubation of reactions at 4 °C. (F) A pre-pandemic human serum was used as a negative control. (G) A positive control was human serum containing 100 ng of the rabbit antibodies against SARS-CoV2 N nucleocapsid protein.

Article Snippet: Rabbit polyclonal antibodies against SARS-CoV2 N nucleocapsid proteins were purchased from SinoBiological (cat. 40588-T62).

Techniques: Labeling, Fluorescence, Incubation, Negative Control, Positive Control

Journal: PeerJ

Article Title: A novel one-step quick assay for detection of SARS-COV2 antibodies across mammalian species

doi: 10.7717/peerj.11381

Figure Lengend Snippet: (A) The positive control was human serum containing 100 ng of the rabbit antibodies against SARS-CoV2 N nucleocapsid protein. The negative controls are 4 representative samples (SDBB1-4 (B–E)) from the 50 pre-pandemic human serum samples from San Diego Blood Bank (SDBB). Patient Group A has 20 SARS-CoV2 patient sera deemed positive (either IgG or IgM positive) by LFIA conducted by RayBiotech. All of the 20 patient samples are positive for anti-scN antibodies by the one-step test. (F–J) 5 representatives of the 20 positive samples are shown. Patient Group B has 20 SARS-CoV2 patient serum samples deemed negative (both IgG and IgM are negative) by LFIA conducted by RayBiotech. A total of 19 out of the 20 LFIA negative samples are positive for anti-scN antibodies by the one-step test. (K–O) 5 representatives of the 19 positive samples are shown.

Article Snippet: Rabbit polyclonal antibodies against SARS-CoV2 N nucleocapsid proteins were purchased from SinoBiological (cat. 40588-T62).

Techniques: Positive Control

Journal: PeerJ

Article Title: A novel one-step quick assay for detection of SARS-COV2 antibodies across mammalian species

doi: 10.7717/peerj.11381

Figure Lengend Snippet: (A) A strategy of direct visualization of antigen-antibody reaction via aggregation by protein A/G/L. Antigen is labeled with GFP. Protein A/G/L cross-links Ig Fc and/or light chain of all antibodies (IgG, IgM, IgA, IgE, and IgD) that bind antigen-GFP to form high fluorescence aggregates. Aggregating fluorescent antigen-antibody complexes simultaneously depletes background fluorescence to achieve a high sensitivity. (B) One-Step assay. Rabbit polyclonal antibodies against SARS-CoV2 N nucleocapsid proteins were diluted in human serum. Different amount of the rabbit antibodies (100 ng (C), 10 ng (D), 1 ng (E)) was incubated with the scN-GFP fusion proteins for 5 min to examine the detection limit of the one-step test. Blackwhite images show antigen-antibody aggregates, and green fluorescence of the aggregates is shown side by side. Bar: 100 um. Non-specific background of the antigen-antibody aggregates was examined after 5 day incubation of reactions at 4 °C. (F) A pre-pandemic human serum was used as a negative control. (G) A positive control was human serum containing 100 ng of the rabbit antibodies against SARS-CoV2 N nucleocapsid protein.

Article Snippet: Rabbit polyclonal antibodies against SARS-CoV2 N nucleocapsid protein (cat. 40588-T62; SinoBiological, Beijing, China) were diluted in human serum to a concentration of 100 ng/ul, 10 ng/ul, 1 ng/ul, respectively.

Techniques: Labeling, Fluorescence, Incubation, Negative Control, Positive Control

Journal: PeerJ

Article Title: A novel one-step quick assay for detection of SARS-COV2 antibodies across mammalian species

doi: 10.7717/peerj.11381

Figure Lengend Snippet: (A) The positive control was human serum containing 100 ng of the rabbit antibodies against SARS-CoV2 N nucleocapsid protein. The negative controls are 4 representative samples (SDBB1-4 (B–E)) from the 50 pre-pandemic human serum samples from San Diego Blood Bank (SDBB). Patient Group A has 20 SARS-CoV2 patient sera deemed positive (either IgG or IgM positive) by LFIA conducted by RayBiotech. All of the 20 patient samples are positive for anti-scN antibodies by the one-step test. (F–J) 5 representatives of the 20 positive samples are shown. Patient Group B has 20 SARS-CoV2 patient serum samples deemed negative (both IgG and IgM are negative) by LFIA conducted by RayBiotech. A total of 19 out of the 20 LFIA negative samples are positive for anti-scN antibodies by the one-step test. (K–O) 5 representatives of the 19 positive samples are shown.

Article Snippet: Rabbit polyclonal antibodies against SARS-CoV2 N nucleocapsid protein (cat. 40588-T62; SinoBiological, Beijing, China) were diluted in human serum to a concentration of 100 ng/ul, 10 ng/ul, 1 ng/ul, respectively.

Techniques: Positive Control

Journal: Nature

Article Title: COVID-19 Treatments and Pathogenesis Including Anosmia in K18-hACE2 mice

doi: 10.1038/s41586-020-2943-z

Figure Lengend Snippet: Distribution of SARS-CoV2 antigen in Brain. 4 brains were analyzed at 6dpi. + <++ <+++ Represents Relative density of antigen presence. - No antigen detected. (+) Represents presence of antigen in some samples only. AON-Anterior Olfactory Nucleus. Neuroanatomical Reference: ALLEN Brain Atlas. http://atlas.brain-map.org/atlas?atlas=2#atlas=2&plate=100883770&structure=168&x=7797.5&y=4023.9999997615814&zoom=−3&resolution=10.47&z=5 .

Article Snippet: For SARS-CoV-2 antigen detection, slides were incubated with blocking reagent (10% normal goat serum x 30 minutes) followed by rabbit monoclonal antibody against SARS-CoV2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako) and diaminobenzidine (Dako) as chromogen.

Techniques: